We are also thankful to Taif University Researchers for Supporting with funds from Project number (TURSP -2020/295), Taif university, Taif, Saudi Arabia.Ĭompeting interests: The authors have declared that no competing interests exist.Įnvironmental pollution is a serious hazard for all living things on earth. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.ĭata Availability: All relevant data are within the manuscript.įunding: The research work is supported by the Higher Education Commission of Pakistan (NRPU-8933). Received: FebruAccepted: JPublished: June 30, 2021Ĭopyright: © 2021 Younis et al. PLoS ONE 16(6):Įditor: Shahid Farooq, Harran Üniversitesi: Harran Universitesi, TURKEY (2021) Fourier Transform Infrared Spectroscopy vibrational bands study of Spinacia oleracea and Trigonella corniculata under biochar amendment in naturally contaminated soil. These types of spectra are useful in detecting changes or visibility of functional groups, which are very helpful in supporting biochemical data such as an increase in protein can be detected by more visibility of C-N bands in FTIR spectra.Ĭitation: Younis U, Rahi AA, Danish S, Ali MA, Ahmed N, Datta R, et al. It is concluded that C-N bands are more visible in 5% BC treated spinach and fenugreek than in all other treatments. In fenugreek, additional effects are observed in the FTIR spectra of chlorophyll at the major groups of C = C, C = O and C9 of the ketonic groups, and the vibrational bands are more evident at C-H and N-H of the tetrapyrrole ring. In addition, the C = O bands with Mg and the C9 ketonic group of chlorophyll are observed as peaks at1600 (0%BC), 1650 (3%BC) and 1640, or near to1700 (5%BC) in spinach samples. The data obtained from the current study also shows that leaf chlorophyll can mask or suppress other molecules’ FITR bands, including proteins. The study’s primary goal was to investigate the silent features of infrared (IR) spectra of dried leave samples. For this, dried leaf of Spinacia oleracia (spinach) and Trigonella corniculata (fenugreek) were selected for FTIR spectral study of chlorophyll associated functional groups. The FTIR of these bands is fundamental to the investigation of the effect of biochar (BC) treatment on structural changes in the chlorophyll molecules of both plants that were tested. Fourier transform infrared spectroscopy (FTIR) spectroscopy detects functional groups such as vibrational bands like N-H, O-H, C-H, C = O (ester, amine, ketone, aldehyde), C = C, C = N (vibrational modes of a tetrapyrrole ring) and simply C = N.
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